The current article describes a naturalistic research programme carried out among fishermen during a 6-month expedition to the southern Atlantic Ocean. Stress levels of 81 participants were measured 4 times during the expedition. Social-demographic variables (age, years of experience at sea, job satisfaction, education, current and childhood place of residence, relationship status) were also included in the analysis. Results show that these variables should be considered when analysing stress dynamics among people experiencing long-term work-related isolation.