The nursing profession entails a specific workload related to interacting with patients and their families, fostering relationships with medical professionals, and managing epidemiological hazards. There exists a need for a Polish-language questionnaire for detecting stressors in nursing to be developed and made available, given the abundance of research on stress in nursing and a lack of publicly accessible diagnostic instruments that are psychometrically validated and permit comparative studies. The purpose of this article is to present the Questionnaire of Stressors in Nursing (QSN), a tool used to measure the occurrence and intensity of stressors in nurses’ work. Our aim was to make this tool accessible for every researcher interested in studying occupational stress in nurses. A total of 436 nurses between the ages of 23 and 63 participated in the study using the 24-item pilot version of the questionnaire. The Questionnaire of Stressors in Nursing (QSN) is characterized by satisfactory psychometric properties (reliability, discriminatory power, validity). It can be used to diagnose the type and intensity of stressors experienced in the workplace. 3-factor structure of the Questionnaire of Stressors in Nursing was established, including: Organization and working conditions, Patient and their family, and Interpersonal relations. The Questionnaire of Stressors in Nursing (QSN) can be recommended for use in scientific research in the field of work and organizational psychology, health psychology, in comparative studies of different groups of nurses, and also in applied research on the design of occupational health and safety measures, as well as in professional counseling.